Ifih1 Knockout cell line (Raw 264.7)

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Ifih1 Knockout cell line (Raw 264.7) Order

Ifih1 Knockout cell line (Raw 264.7)
Catalog#
YKO-MT0083
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Ifih1 Knockout cell line (Raw 264.7)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Ifih1 Knockout cell line (Raw 264.7)
Species
Mouse
Gene Name
Ifih1
Gene ID
71586
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript Ifih1-201)
Validation: PCR+Sanger Sequencing
Ifih1 Knockout cell line (Raw 264.7) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Ifih1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Raw 264.7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Ifih1 gene knockout Ifih1 Knockout cell line (Raw 264.7) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Ifih1 Knockout cell line (Raw 264.7)
Ifih1 Knockout cell line (Raw 264.7)
Catalog#
YKO-MT0083
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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    Catalog#: YKO-HT0241
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      inquiry   More details

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    YKO-H825(293T)
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    Gene Name: IFIH1
    Gene ID: 64135
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    Catalog#: YKO-HS14312
    YKO-HS14312(HeLa)

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  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • IFIH1 Knockout cell line (THP-1)

    IFIH1 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: IFIH1
    Gene ID: 64135
    Size: 1*10^6
    Catalog#: YKO-HT0241
      inquiry   More details
  • IFIH1 Knockout cell line (293T)

    IFIH1 Knockout cell line (293T)

    Organism: Human
    Gene Name: IFIH1
    Gene ID: 64135
    Size: 1*10^6
    Catalog#: YKO-H825
      inquiry   More details
  • IFIH1 Knockout cell line (HeLa)

    IFIH1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: IFIH1
    Gene ID: 64135
    Size: 1*10^6
    Catalog#: YKO-HS14312
      inquiry   More details

Ifih1 Knockout cell line (Raw 264.7) Order

Ifih1 Knockout cell line (Raw 264.7)
Catalog#
YKO-MT0083
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Ifih1 Knockout cell line (Raw 264.7)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Ifih1 Knockout cell line (Raw 264.7)
Species
Mouse
Gene Name
Ifih1
Gene ID
71586
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript Ifih1-201)
Validation: PCR+Sanger Sequencing
Ifih1 Knockout cell line (Raw 264.7) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Ifih1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Raw 264.7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Ifih1 gene knockout Ifih1 Knockout cell line (Raw 264.7) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Ifih1 Knockout cell line (Raw 264.7)
Ifih1 Knockout cell line (Raw 264.7)
Catalog#
YKO-MT0083
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • IFIH1 Knockout cell line (THP-1)

    IFIH1 Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: IFIH1
    Gene ID: 64135
    Size: 1*10^6
    Catalog#: YKO-HT0241
    YKO-HT0241(THP-1)
  • IFIH1 Knockout cell line (293T)

    IFIH1 Knockout cell line (293T)
      inquiry   More details

    Organism: Human
    Gene Name: IFIH1
    Gene ID: 64135
    Size: 1*10^6
    Catalog#: YKO-H825
    YKO-H825(293T)
  • IFIH1 Knockout cell line (HeLa)

    IFIH1 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: IFIH1
    Gene ID: 64135
    Size: 1*10^6
    Catalog#: YKO-HS14312
    YKO-HS14312(HeLa)

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Wild-type Cell Lines

  • RAW 264.7 cell line

    Mouse Macrophages transformed by leukemia virus(RAW 264.7)

    Price (USD): -
    Catalog#: YC-C020
    Size: 1*10^6
    Instruction: Mouse Macrophages transformed by leukemia virus(RAW 264.7)
      inquiry   More details

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • IFIH1 Knockout cell line (THP-1)

    IFIH1 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: IFIH1
    Gene ID: 64135
    Size: 1*10^6
    Catalog#: YKO-HT0241
      inquiry   More details
  • IFIH1 Knockout cell line (293T)

    IFIH1 Knockout cell line (293T)

    Organism: Human
    Gene Name: IFIH1
    Gene ID: 64135
    Size: 1*10^6
    Catalog#: YKO-H825
      inquiry   More details
  • IFIH1 Knockout cell line (HeLa)

    IFIH1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: IFIH1
    Gene ID: 64135
    Size: 1*10^6
    Catalog#: YKO-HS14312
      inquiry   More details

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